OPTIMZATION OF ENZYMATIC HYDROLYSIS AND BIOETHANOL FERMENTATION USING RICE STRAW AS A SUBSTRATE

OPTIMZATION OF ENZYMATIC HYDROLYSIS AND BIOETHANOL FERMENTATION USING RICE STRAW AS A SUBSTRATE

Bambang Dwi Argo¹, J. Bambang Rahadi W.¹, Poppy Diana Sari^1*

¹ Department of Agricultural Engineering, Faculty of Agricultural Technology, Brawijaya University, Malang – Indonesia.

*Corresponding author. Email : p.diana.sari@gmail.com

ABSTRACT

Bioethanol is the result of glucose fermentation. Due to the high demand of glucose, the fuel is transferred to the used of lignocellulosic material, where lignocellulosic materials can be processed into bioethanol by hydrolysis and fermentation. The objective of this research was to obtain the optimal conditions of hydrolysis and fermentation. The first phase was hydrolysis, carried out with two factors, the first was a blend of enzymes cellulase from Trichoderma reesei and Aspergillus niger by ratio of 1:0, 0:1, 1:1, 1:2, 1:3, 2:1 and 3:1 (unit/unit), the second was the hydrolysis time, observations was done every 8 hours for 72 hours. The second phase was fermentation, carried out with two factors, the first was the hydrolyzed solution ph of pH 4, 5 and 6, the second was the amount of Saccharomyces cerevisiae inoculums of 0.5% and 0.75%.

The optimal conditions of enzyme combination from Trichoderma reesei and Aspergillus niger for hydrolysis is 3:1 derived optimal hydrolysis time of 48.7 hours produce an optimal glucose and D-optimal value was 10% and 1.0000, respectively. While at the fermentation phase, ethanol derived optimal level and D-optimal value was 5.5% and 1.0000, respectively at pH 3.59 and 0.7462% of Saccharomyces cerevisiae inoculum.

Key words: Optimization, Hydrolysis, glucose, fermentation, Saccharomyces cerevisiae, ethanol. 

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EFFECT OF PH AND INCUBATION TIME AGAINST ENDOGLUCANASE ACTIVITY

EFFECT OF PH AND INCUBATION TIME AGAINST Endoglucanase ACTIVITY FROM Trichoderma reesei AND Aspergillus niger WITH RICE STRAW AS SUBSTRAT

Bambang Dwi Argo¹, J. Bambang Rahadi W.¹, Poppy Diana Sari^1*

¹ Department of Agricultural Engineering, Faculty of Agricultural Technology, Brawijaya University, Malang – Indonesia.

*Corresponding author. Email : p.diana.sari@gmail.com

ABSTRACT

Endoglucanase is one important part of the enzyme cellulase, which works to solve bonding polymer β-1,4 which is a part of the amorphous cellulose, and produce cello-oligosaccharides. Analysing Carboxy Methyl Cellulase enzyme activity can reflect the Endoglucanase activity contained in cellulase enzyme. This study aimed to determine the effect of pH and incubation time on the activity of the enzyme cellulase Endoglucanase from Trichoderma Reesei and Aspergillus Niger. The research divided into two phases, each phases was done with 2 variables namely pH and incubation time. pH 4, 5 and 6 for the 1^st phase and pH X, pH X.2, pH X.4, pH X.5, pH X.6, and pH X.8 for the 2^nd phase. The value of X in the second phase of treatment is the pH value of the first phase which produced the highest Endoglucanase activity. Observation was done for 10 days and were made every 24 hours. Each treatment performed with 3 replicates and treatments carried out with 2 different types of fungus.

The pH level and incubation time affects the activity of endo-β1,4-glucanase  on the enzyme cellulase. The optimum pH condition of cellulose enzyme production with a high endo-β1,4-glucanase  produced by Trichoderma Reesei is at range of pH 4.5 to pH 6 with an optimum incubation time of 3 to 10 days. While the optimum pH condition of celluloase enzyme production with high endo-β1,4-glucanase  produced by Aspergillus Niger is at range of pH 5 to pH 5.6 with an optimum incubation time of 8 to 10 days.

Keywords: Endoglucanase, Trichoderma Reesei, Aspergillus Niger, pH, incubation time

If need the full version of the journal, please contact us by email to p.diana.sari@gmail.com