OPTIMZATION OF ENZYMATIC HYDROLYSIS AND BIOETHANOL FERMENTATION USING RICE STRAW AS A SUBSTRATE

OPTIMZATION OF ENZYMATIC HYDROLYSIS AND BIOETHANOL FERMENTATION USING RICE STRAW AS A SUBSTRATE

Bambang Dwi Argo¹, J. Bambang Rahadi W.¹, Poppy Diana Sari^1*

¹ Department of Agricultural Engineering, Faculty of Agricultural Technology, Brawijaya University, Malang – Indonesia.

*Corresponding author. Email : p.diana.sari@gmail.com

ABSTRACT

Bioethanol is the result of glucose fermentation. Due to the high demand of glucose, the fuel is transferred to the used of lignocellulosic material, where lignocellulosic materials can be processed into bioethanol by hydrolysis and fermentation. The objective of this research was to obtain the optimal conditions of hydrolysis and fermentation. The first phase was hydrolysis, carried out with two factors, the first was a blend of enzymes cellulase from Trichoderma reesei and Aspergillus niger by ratio of 1:0, 0:1, 1:1, 1:2, 1:3, 2:1 and 3:1 (unit/unit), the second was the hydrolysis time, observations was done every 8 hours for 72 hours. The second phase was fermentation, carried out with two factors, the first was the hydrolyzed solution ph of pH 4, 5 and 6, the second was the amount of Saccharomyces cerevisiae inoculums of 0.5% and 0.75%.

The optimal conditions of enzyme combination from Trichoderma reesei and Aspergillus niger for hydrolysis is 3:1 derived optimal hydrolysis time of 48.7 hours produce an optimal glucose and D-optimal value was 10% and 1.0000, respectively. While at the fermentation phase, ethanol derived optimal level and D-optimal value was 5.5% and 1.0000, respectively at pH 3.59 and 0.7462% of Saccharomyces cerevisiae inoculum.

Key words: Optimization, Hydrolysis, glucose, fermentation, Saccharomyces cerevisiae, ethanol. 

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