EFFECT OF PH AND INCUBATION TIME AGAINST Endoglucanase ACTIVITY FROM
Trichoderma reesei AND Aspergillus niger WITH RICE STRAW AS SUBSTRAT
Bambang Dwi Argo1,
J. Bambang Rahadi W.1, Poppy Diana Sari1*
1 Department
of Agricultural Engineering,
Faculty of Agricultural Technology,
Brawijaya University, Malang –
Indonesia.
ABSTRACT
Endoglucanase is one important part of the
enzyme cellulase, which works to solve bonding polymer β-1,4 which is a part of
the amorphous cellulose, and produce cello-oligosaccharides. Analysing Carboxy Methyl
Cellulase enzyme activity can reflect the Endoglucanase
activity contained in cellulase enzyme. This
study aimed to determine the
effect of pH and incubation time on the activity of the enzyme cellulase Endoglucanase from Trichoderma
Reesei and Aspergillus Niger.
The research divided into
two phases, each phases was done with 2 variables namely pH and incubation
time. pH 4, 5 and 6 for the 1st phase and pH X, pH X.2, pH X.4, pH
X.5, pH X.6, and pH X.8 for the 2nd phase. The value of X in the
second phase of treatment is the pH value of the first phase which produced the
highest Endoglucanase activity. Observation was done for 10 days and were made
every 24 hours. Each treatment performed with 3 replicates and treatments
carried out with 2 different types of fungus.
The pH level and
incubation time affects the activity of endo-β1,4-glucanase on the enzyme cellulase. The optimum pH
condition of cellulose enzyme production with a high endo-β1,4-glucanase produced by Trichoderma Reesei is at range of
pH 4.5 to pH 6 with an optimum incubation time of 3 to 10 days. While the
optimum pH condition of celluloase enzyme production with high endo-β1,4-glucanase
produced by Aspergillus Niger is at
range of pH 5 to pH 5.6 with an optimum incubation time of 8 to 10 days.
Keywords: Endoglucanase, Trichoderma
Reesei, Aspergillus Niger, pH, incubation time
If need the full version of the journal, please contact us by email to p.diana.sari@gmail.com
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