OPTIMZATION
OF ENZYMATIC HYDROLYSIS AND BIOETHANOL FERMENTATION USING RICE STRAW AS A
SUBSTRATE
Bambang
Dwi Argo1, J. Bambang Rahadi W.1, Poppy Diana Sari1*
1 Department
of Agricultural Engineering,
Faculty of Agricultural Technology,
Brawijaya University, Malang –
Indonesia.
ABSTRACT
Bioethanol is the
result of glucose fermentation. Due
to the high demand of glucose,
the fuel is transferred
to the used of lignocellulosic material, where lignocellulosic materials can be processed into bioethanol by hydrolysis and fermentation. The objective of this research was to obtain the optimal conditions
of hydrolysis and fermentation. The first phase was hydrolysis, carried out with two factors, the first
was a blend of enzymes
cellulase from Trichoderma reesei and Aspergillus niger by ratio of 1:0, 0:1,
1:1, 1:2, 1:3,
2:1 and 3:1
(unit/unit), the second was the
hydrolysis time, observations was
done every 8 hours for 72 hours. The second phase was fermentation, carried out with two factors,
the first was the hydrolyzed solution ph
of pH 4, 5
and 6, the
second was the amount
of Saccharomyces cerevisiae inoculums of 0.5% and
0.75%.
The optimal
conditions of enzyme combination from Trichoderma reesei and Aspergillus
niger for hydrolysis is 3:1 derived optimal hydrolysis time of 48.7 hours produce an optimal glucose and D-optimal
value was 10% and 1.0000, respectively. While
at the fermentation phase, ethanol
derived optimal level
and D-optimal value was 5.5% and 1.0000,
respectively at pH 3.59 and 0.7462% of Saccharomyces cerevisiae inoculum.
Key words: Optimization,
Hydrolysis, glucose, fermentation, Saccharomyces cerevisiae,
ethanol.
If need the full version of this journal, please contact us at p.diana.sari@gmail.com
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